Denitrifying Dephosphatation Performance Link to Microbial Community Structure

Wen-De Tian, Wei-Guang Li, Kyoung-Jin An,Xiao-Rong Kang, Cong Ma, Zhi-Lin Ran



In order to investigate the dynamic relationship of microbial community structure and denitrifying dephosphatation performance of the process, fingerprint profile technique of polymerase chain reaction (PCR) amplified 16S rRNA and denaturing gradient gel electrophoresis (DGGE) were applied to analyze microbial communities in anaerobic/anoxic/oxic enhanced biological phosphorus removal (EBPR) process. The chemical analysis showed that average removal efficiency of COD, NH4+–N and TP was 90%, 90% and 97% respectively during the steady-state phase. Herein, about 40% of total phosphorus (TP) was removed by denitrifying dephosphatation. Phylogenetic affiliation of predominant members was assessed by the determination of 16S rRNA sequence of correspondent bands. The comparison of these gradual shifts and removal performance of the process suggested that Bacteroidetes was positive for EBPR, while α-proteobacteria was related to EBPR deterioration. Moreover, Uncultured Sphingobacteriaceae was likely to be one of the denitrifying phosphate accumulating organisms (DPAOs), which could contribute to denitrifying dephosphatation.


Keywords: Denitrifying dephosphatation; Removal performance; DGGE; Microbial community structure; EBPR