Phenol Degradation in Aqueous Solutions by Pseudomonas sp. Isolated from Contaminated Soil of Mining Industry

Mahendra Aryal, Maria Liakopoulou-Kyriakides



Batch experiments were performed for different initial phenol concentrations in the presence of glucose to observe cell growth and associated phenol degradation using Pseudomonas sp. cells. It was observed that phenol degradation increases with increasing the tolerance of the cells to phenol inhibition using glucose in the growth medium. The maximum phenol biodegradation was achieved at pH 7.0, glucose concentration 1.0 g/L and incubation temperature 30oC, respectively. The specific growth rate of Pseudomonas sp. increases with increase in initial phenol concentration ranging from 10 to 25 mg/L and then decreases to the initial phenol concentration followed to 300 mg/L. Haldane growth kinetic model fitted better experimental data than Aiba and Edwards models. It was also found that removal efficiency of Pseudomonas sp. for phenol decreases from 51.5 to 9.5% with increase in initial phenol concentration from 10 to 300 mg/L at 24 h. The results showed that Pseudomonas sp. can be used to remove the phenol from industrial waste waters.